A specific nanoprobe for cysteine based on nitrogen-rich fluorescent quantum dots combined with Cu2+

Publication date: 15 February 2018
Source:Biosensors and Bioelectronics, Volume 100
Author(s): Tingting Gu, Wu Zou, Fuchun Gong, Jiaoyun Xia, Can Chen, Xuejiao Chen
As a new member of the carbon quantum-dot family, fluorescent nitrogen-rich quantum dots (NRQDs) were prepared by a mixed solvothermal method using 2-azidoimidazole and aqueous ammonia as reactants. These NRQDs are rich in nitrogen up to 40.2%, which are endowed with high fluorescence quantum yield, good photostability, water-solubility and favourable biocompatibility. We further explored the use of NRQDs combined with Cu²⁺ as a nanoprobe for sensing fluorescently of cysteine (Cys) in complex biological samples. In this sensing system, the fluorescence is significantly quenched via energy transfer from NRQDs to Cu²⁺ for the coordination of amino-containing groups with Cu²⁺. The strong affinity between Cu ²⁺ and Cys leads to the formation of Cu²⁺-Cys complexes and cause the detachment of Cu²⁺ from the surface of NRQDs, thus the fluorescence of NRQDs recover. This nanoprobe allows analysis of Cys by modulating the switch of the fluorescence of NRQDs with a detection limit of 5.3nM. As expected, the proposed NRQDs-Cu²⁺complex-based nanoprobes were successfully applied for the determination of Cys in human serum and plasma samples with recoveries ranging from 97.2% to 105.7%. The probe ensemble was also successfully applied to imaging of Cys in living cells with satisfactory results, which shows strong potential for clinical diagnosis.



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