CRISPR/Cas9-Based Engineering of the Epigenome

Publication date: 5 October 2017
Source:Cell Stem Cell, Volume 21, Issue 4
Author(s): Julian Pulecio, Nipun Verma, Eva Mejía-Ramírez, Danwei Huangfu, Angel Raya
Determining causal relationships between distinct chromatin features and gene expression, and ultimately cell behavior, remains a major challenge. Recent developments in targetable epigenome-editing tools enable us to assign direct transcriptional and functional consequences to locus-specific chromatin modifications. This Protocol Review discusses the unprecedented opportunity that CRISPR/Cas9 technology offers for investigating and manipulating the epigenome to facilitate further understanding of stem cell biology and engineering of stem cells for therapeutic applications. We also provide technical considerations for standardization and further improvement of the CRISPR/Cas9-based tools to engineer the epigenome.

Teaser

Pulecio et al. discuss opportunities that CRISPR/Cas9 technology offers for investigating and manipulating the stem cell epigenome and also provide technical considerations for CRISPR/Cas9 tool standardization and improvement.


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