Protective effect of α–lipoic acid against spleen toxicity of dimethylnitrosamine in male mice: Antioxidant and ultrastructure approaches

Publication date: December 2017
Source:Biomedicine & Pharmacotherapy, Volume 96
Author(s): Nahla S. El-Shenawy, Reham Z. Hamza, Howayda E. Khaled
The α–lipoic acid is a soft material and useful for the potential biomedical applications. The study was aimed to assess the potency of α-lipoic acid (ALA) against the toxicity of dimethylnitrosamine (DMN) on spleen of mice by assessing the antioxidants, histopathological and ultrastructure changes. The experiment was achieved on six groups of male mice as following; groups 1, 2, 3, and 4 were served as a control, ALA groups, low dose of DMN (DMN-LD; 2mgkg⁻¹) and high dose of DMN (DMN-HD; 4mgkg⁻¹). Group 5 was received DMN-LD plus ALA and group 6 was given DMN-HD plus ALA. The results indicated that DMN elevated lipid peroxidation, xanthine oxidase, nitric oxide, and decline the antioxidant enzymes as well as raise the C-reactive protein and tumor necrosis factor. A critical obstruction was harmonized with a reduced in lymphocyte number in the white pulp were observed. All the lymphatic nodules appeared smaller in DMN-HD group. In spleen tissues, marked changes of rough endoplasmic reticulum and appearance of three large lymphocytes were noticed. ALA/DMN treatments were improved all the oxidative damage and the ultrastructure changes. The data evince that ALA was eliminated the adverse effects of DMN on spleen of mice.



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