Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor

Publication date: 15 April 2018
Source:Biosensors and Bioelectronics, Volume 102
Author(s): Ying Chen, Yu Li, Dongmei Deng, Haibo He, Xiaoxia Yan, Zhenxin Wang, Chunhai Fan, Liqiang Luo
A novel and sensitive sandwich immunosensor for amperometric determination of carcinoembryonic antigen (CEA) was designed using Au nanoparticles-titanium dioxide-graphene (AuNPs-TiO2-graphene) nanocomposites. Dopamine-functionalized graphene was firstly prepared by π-stacking interaction, and TiO2 was then attached to the surface of dopamine-graphene by the specificity and high affinity of enediol ligands to Ti (IV). Afterwards, AuNPs-TiO2-graphene nanocomposites were synthesized with photo-reduction approach under ultraviolet irradiation. The morphology and conductivity of the as-prepared nanocomposites were characterized by transmission electron microscopy, Fourier transform infrared spectra, X-ray powder diffraction, cyclic voltammetry and electrochemical impedance spectroscopy. Taking the advantage of large specific surface area and excellent biocompatibility, AuNPs could covalently link horseradish peroxidase labeled secondary antibody (HRP-Ab2) through the interaction between AuNPs and mercapto or primary amine groups of HRP-Ab2 for sandwich-type immunosensor construction. Under optimum conditions, the modified electrode exhibited a linear current response to CEA concentration in a wide range of 0.005–200ngmL⁻¹ (R² = 0.994) with low detection limit of 3.33pgmL⁻¹ (S/N = 3).



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