The effects of Icariin on wound healing of extraction sites with administration of Zoledronic and Dexamethasone: A rat model study

Abstract

Objective

This study was intended to investigate the effects of Icariin on the healing of tooth extraction sites under systemic administration of Zoledronic and Dexamethasone.

Method

Thirty female rats underwent bilateral ovariectomy and were randomly assigned to 5 groups: SS group received weekly injection of saline while ZD, ZD+LICA, ZD+MICA and ZD+HICA groups received Zoledronic with Dexamethasone for 8 weeks. One week later, mandibular first molars were extracted in all groups. Then 30, 60, 120mg/kg Icariin were intragastricly given to ZD+LICA, ZD+MICA and ZD+HICA groups daily for 10 weeks while saline was given to SS group and ZD group. Blood samples and mandibles were harvested for examinations after 10 weeks.

Results

Significantly smaller wound area was noted in SS and ZD+HICA groups but the incidence of bisphosphonates-related osteonecrosis of the jaws (BRONJ) was not significantly different. Groups injected with Zoledronic and Dexamethasone had higher C-terminal crosslinked telopeptide of type 1 collagen (CTX-1), tartrate-resistant acid phosphatase 5b (TRACP 5b) and the number of osteoclast cells, with less vascular endothelial growth factor (VEGF) and osteocalcin (OCN). In contrast, CTX-1, TRACP 5b and the number of osteoclast cells declined after using Icariin and promoted VEGF and OCN were noted; and the effects were in a dosage-dependent manner.

Conclusion

Concurrent use of Zoledronic and Dexamethasone inhibits expression of VEGF, OCN and wound healing; and increases the number of osteoclast cells, serum CTX-1 and TRACP-5b after discontinuation for ten weeks. Icariin weakens those effects at a dose-dependent manner but does not influence onset of BRONJ.

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