Long non-coding RNA XLOC_008466 acts as an oncogenic molecular in cervical cancer tumorigenesis

Publication date: February 2018
Source:Biomedicine & Pharmacotherapy, Volume 98
Author(s): Fang Guo, Yun-Zhi Chen, Liu Li, Cong Chen, Jian-Hua Jin, Jie Yang, Jing-Jing Chen, Xin-Yan Chen, Min Guo, Yu-Mei Chen
Cervical cancer is one of the most common malignant carcinomas in the female reproductive system. Long non-coding RNAs (lncRNAs) have been verified to participate in the tumorigenesis of cervical cancer. In present study, we investigate the role of lncRNA XLOC_008466 in the occurrence and progression of cervical cancer. Results showed that XLOC_008466 expression was up-regulated in cervical cancer tissue and cells compared to normal controls. In vitro functional experiments, CCK-8 assay and colony formation assay showed that XLOC_008466 knockdown suppressed the proliferation of cervical cancer cells. Flow cytometry and transwell assay showed that XLOC_008466 knockdown induced G0/G1 phase arrest and aggravated the apoptosis. In vivo, XLOC_008466 knockdown inhibited the tumor growth. Bioinformatics analysis revealed that XLOC_008466 sponged miR-216b with the complementary binding sites at 3'-UTR. Overall, our study reveals the tumor promoting role of XLOC_008466 in cervical cancer carcinogenesis, providing a novel molecular mechanism and therapeutic target for cervical cancer.



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