Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis.

Targeting of miR-31/96/182 to the Numb gene during head and neck oncogenesis.

Head Neck. 2018 Jan 22;:

Authors: Chou CH, Tu HF, Kao SY, Chiang CF, Liu CJ, Chang KW, Lin SC

Abstract
BACKGROUND: MicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR-31, miR-96, and miR-182 are involved in targeting Numb during HNSCC.
METHODS: The expression of miR-31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines.
RESULTS: Upregulation of miR-31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients (P < .01 or P < .001). These miRNAs directly targeted the 3' untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR-31/96/182 (P < .01 or P < .001), and this was reverted by expression of Numb.
CONCLUSION: This study provides new evidence demonstrating that there is frequent and concordant upregulation of miR-31, miR-96, and miR-182 during HNSCC and these miRNAs co-target Numb.

PMID: 29356167 [PubMed - as supplied by publisher]

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