Two novel microRNA biomarkers related to β-cell damage and their potential values for early diagnosis of type 1 diabetes.

Two novel microRNA biomarkers related to β-cell damage and their potential values for early diagnosis of type 1 diabetes.

J Clin Endocrinol Metab. 2018 Jan 23;:

Authors: Liu L, Yan J, Xu H, Zhu Y, Liang H, Pan W, Yao B, Han X, Ye J, Weng J

Abstract
Context: New strategies and biomarkers are needed in the early detection of β-cell damage in the progress of type 1 diabetes mellitus (T1DM).
Objective: To explore whether serum microRNAs should be served as biomarkers for T1DM.
Design, Settings and Patients: The serum microRNA profile was established with microRNA microarray in discovery phase (6 T1DM, 6 controls). A microRNA-based model for T1DM diagnosis was developed using logistic regression analysis in the training dataset (40 T1DM, 56 controls) and then validated with leave-one-out cross validation and another independent validation dataset (33 T1DM, 29 controls).
Main Outcome Measure(s): Quantitative RT-PCR was applied to confirm the differences of candidate microRNAs between T1DM and controls. Area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy. INS-1 cells, streptozocin-treated mice (n=4) and NOD mice (n=12) were used to evaluate the association of microRNAs with β-cell damage.
Results: A microRNA-based model was established in the training dataset with high diagnostic accuracy for T1DM (AUC=0.817) based on six candidate differential expressed microRNAs identified in discovery phase. The validation dataset showed the model's satisfactory diagnostic performance (AUC=0.804). Secretions of miR-1225-5p and miR-320c were significantly increased in streptozocin-treated mice and INS-1 cells. Noteworthy, the elevation of these two microRNAs was observed before glucose elevation in the progress of diabetes in NOD mice.
Conclusions: Two microRNA biomarkers (miR-1225-5p and miR-320c) related to β-cell damage were identified in recent-onset T1DM patients. The microRNA-based model established in this study exhibited a good performance in diagnosis of T1DM.

PMID: 29370422 [PubMed - as supplied by publisher]

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