Vitamin D modulatory effect on cytokines expression by human gingival fibroblasts and periodontal ligament cells.

Vitamin D modulatory effect on cytokines expression by human gingival fibroblasts and periodontal ligament cells.

Minerva Stomatol. 2018 Feb 09;:

Authors: Nastri L, Guida L, Annunziata M, Ruggiero N, Rizzo A

Abstract
BACKGROUND: It has been demonstrated that vitamin D exerts several functions other than those implied in the bone homeostasis. It has been published the vitamin D can act on many cells and tissues behaving also as a modulatory factor in the immune responses.
OBJECTIVE: The aim of our study was to evaluate the effect of active vitamin D3 (VD) on the expression of pro-inflammatory and anti-inflammatory cytokines (IL-6, IL-8, IL- 10 and IL-12) in human Gingival Fibroblasts (hGF) and human Periodontal Ligament cells (hPDLc) triggered by Porphyromonas gingivalis and Streptococcus pyogenes.
METHODS: Primary hGF and hPDLc pre-treated or not by VD (10-8 mol/l) were exposed to P. gingivalis and S. progenies for 24h. Production of IL-6, IL-8, IL-10 and IL-12 was evaluated by immunoenzymatic assay. mRNA of the same cytokines were evaluated by PCR.
RESULTS: IL-6 secretion increased by 25.2%(±2.1) up to 51%(±3.3) in VD treated hGF and hPDLc exposed to P. gingivalis and S. pyogenes, compared to VD not treated cells. IL-8 secretion decreased approximately by 30% in VD-treated hGF and hPDLc compared to VD not-treated cells. IL-12 secretion decreased by 60%. On the contrary, anti-inflammatory cytokine IL-10 increased by approximately 200%. mRNA PCR confirmed these results.
CONCLUSIONS: Within the limits of the study, the obtained results support the hypothesis of a modulatory role of VD on periodontal cells exposed to bacterial infection, reducing their inflammatory response and increasing the secretion of anti-inflammatory and modulatory cytokines. Consequently, it could be speculated that Vitamin D assessment, and its possible implementation in deficiency cases, could play a role in periodontal treatment.

PMID: 29431349 [PubMed - as supplied by publisher]

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