Enteroviral Infection Inhibits Autophagic Flux via Disruption of the SNARE Complex to Enhance Viral Replication

Publication date: 20 March 2018
Source:Cell Reports, Volume 22, Issue 12
Author(s): Yasir Mohamud, Junyan Shi, Junyan Qu, Tak Poon, Yuan Chao Xue, Haoyu Deng, Jingchun Zhang, Honglin Luo
Picornaviruses have evolved to hijack host cellular machinery, including the autophagic pathway. However, the mechanisms remain largely unclear. We use coxsackievirus B3 (CVB3) as a model organism to explore the possible role of picornavirus subversion of the autophagic pathway in viral infection. Our in vivo and in vitro experiments demonstrate that CVB3 infection causes a significant, albeit incomplete, inhibition of autophagic flux by limiting the fusion of autophagosomes with lysosomes and/or late endosomes. Furthermore, we show that CVB3 specifically targets SNARE protein SNAP29 and adaptor protein PLEKHM1, two critical proteins known to regulate autophagosome fusion, for cleavage through the catalytic activity of viral proteinase 3C, ultimately impairing the formation of SNARE complexes. Finally, we demonstrate that loss of SNAP29/PLEKHM1 inhibits autophagic flux, resulting in increased viral replication. Collectively, our study reveals a mechanism that supports an emerging model whereby CVB3 hijacks the autophagic machinery to facilitate its own propagation.

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Teaser

Mohamud et al. report that coxsackievirus limits autophagosome fusion with lysosomes and/or late endosomes through viral proteinase 3C-mediated cleavage of SNAP29 and PLEKHM1. This leads to the accumulation of autophagosomes that favor viral replication by providing additional membrane scaffolds.


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