Highly sensitive detection of hepatitis C virus DNA by using a one-donor-four-acceptors FRET probe

Publication date: 1 August 2018
Source:Talanta, Volume 185
Author(s): Ping Zeng, Peng Hou, Chun Ju Jing, Cheng Zhi Huang
Sensitive detection of Hepatitis C virus (HCV) DNA is critical to treat and prevent the propagation of hepatitis C diseases. Herein a FRET probe of One-Donor-Multi-Acceptors (D–mA) assembly is designed through the formation of triple-helix DNA structure, which can greatly reduce the background of FRET system so as to greatly increase the detection sensitivity of hepatitis C virus DNA with the limit of detection (3σ) being 24.57 nmol/L, making the detection of clinic samples successful and showing that the newly developed FRET probe of D–mA assembly could be applied for the early diagnosis and treatment of hepatitis C disease.

Graphical abstract

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