Deep sequencing of small RNAs in blood of patients with brain arteriovenous malformations.

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Deep sequencing of small RNAs in blood of patients with brain arteriovenous malformations.

World Neurosurg. 2018 Apr 21;:

Authors: Chen Y, Li Z, Shi Y, Huang G, Chen L, Tan H, Wang Z, Yin C, Hu J

Abstract
BACKGROUND: The deregulation of circulating microRNAs (miRNAs) is always associated with development and progression of human diseases. We are aimed to assess whether brain arteriovenous malformations (BAVMs) patients present a distinct miRNA signature compared with healthy subjects.
METHODS: Three unruptured BAVMs patients and three normal controls were recruited as case and normal groups. Peripheral blood was collected and miRNA signature was obtained by next-generation sequencing, followed by comparative, functional, and network analyses. Further qRT-PCR was performed to validate the expression of specific miRNAs.
RESULTS: Deep sequencing detected 246 differentially expressed miRNAs in blood samples of BAVM patients compared with normal controls. For the top five miRNAs, 946 target genes were predicted and a BAVM-specific miRNA-target gene regulatory network was then constructed. Functional annotation suggested that 15 of the predicted miRNAs targeted genes were involved in VEGF signaling, in which three critical miRNAs involved, including miR-7-5p, miR-199a-5p and miR-200b-3p.
CONCLUSION: We explored the miRNA expression signature of BAVMs, which will provide an important foundation for future studies on the regulation of miRNAs involved in BAVMs.

PMID: 29689389 [PubMed - as supplied by publisher]

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