Molecular allergy diagnostics refines characterization of children sensitized to dog dander

Publication date: Available online 29 May 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Ulrika Käck, Anna Asarnoj, Hans Grönlund, Magnus P. Borres, Marianne van Hage, Gunnar Lilja, Jon R. Konradsen
BackgroundSensitization to dog dander is an important risk factor for rhino-conjunctivitis and asthma, but not sufficient for diagnosing dog allergy. Molecular allergy diagnostics offers new opportunities for refined characterization.ObjectivesTo study the association between sensitization to all presently known dog allergen components and clinical symptoms of dog allergy in children, evaluated by nasal provocation testing.Methods60 children (age 10-18 years) sensitized to dog dander extract, went through nasal provocation testing with dog dander extract. Measurement of IgE to dog dander and to Can f 1, Can f 2, Can f 3 and Can f 5 was performed with ImmunoCAP and to Can f 4 and Can f 6 with Streptavidin ImmunoCAP. An IgE level ≥ 0.1 kUA/L was considered positive.ResultsThere was an association between sensitization to an increasing number of dog allergen components and a positive nasal challenge (p=0.01). Sensitization to lipocalins (OR 6.0, CI 1.04-34.5), in particular Can f 4 (OR 6.80, CI 1.84-25.2) and Can f 6 (OR 5.69, OR 1.59-20.8) was associated with a positive nasal provocation test.Mono-sensitization to Can f 5 was related to a negative nasal provocation test (OR 5.78, CI 1.01-33.0).ConclusionSensitization an increasing number of dog allergen components, as well as sensitization to lipocalins is associated with dog allergy. Mono-sensitization to Can f 5 should not primarily be regarded as a marker for dog allergy.

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Molecular diagnostics refines characterization of dog dander sensitized children. The clinical impact of sensitization to dog allergen components was here for the first time evaluated by nasal provocation testing with dog dander extract.


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