Nano-biosensor for highly sensitive detection of HER2 positive breast cancer

Publication date: 15 October 2018
Source:Biosensors and Bioelectronics, Volume 117
Author(s): Razieh Salahandish, Ali Ghaffarinejad, Seyed Morteza Naghib, Keivan Majidzadeh-A, Hossein Zargartalebi, Amir Sanati-Nezhad
Nanocomposite materials have provided a wide range of conductivity, sensitivity, selectivity and linear response for electrochemical biosensors. However, the detection of rare cells at single cell level requires a new class of nanocomposite-coated electrodes with exceptional sensitivity and specificity. We recently developed a construct of gold nanoparticle-grafted functionalized graphene and nanostructured polyaniline (PANI) for high-performance biosensing within a very wide linear response and selective performance. Further, replacing the expensive gold nanoparticles with low-cost silver nanoparticles as well as optimizing the nanocomposite synthesis and functionalization protocols on the electrode surface in this work enabled us to develop ultrasensitive nanocomposites for label-free detection of breast cancer cells. The sensor presented a fast response time of 30 min within a dynamic range of 10 − 5 × 10⁶ cells mL⁻¹ and with a detection limit of 2 cells mL⁻¹ for the detection of SK-BR3 breast cancer cell. The nano-biosensor, for the first time, demonstrated a high efficiency of > 90% for the label-free detection of cancer cells in whole blood sample without any need for sample preparation and cell staining. The results demonstrated that the optimized nanocomposite developed in this work is a promising nanomaterial for electrochemical biosensing and with the potential applications in electro-catalysis and super-capacitances.



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