Xiaokeping-induced autophagy protects pancreatic β-cells against apoptosis under high glucose stress

Publication date: September 2018
Source:Biomedicine & Pharmacotherapy, Volume 105
Author(s): Yanyang Wu, Yongquan Hu, Zhou Haiyan, Wei YunLin, Kang Xincong, Liu Dongbo
Xiaokeping (XKP), a prescribed Traditional Chinese Medicine (TCM), has been used to treat patients with type Ⅱ diabetes mellitus for many years; however, the molecular mechanism of its effects is unknown. As the only insulin producer, the pancreatic β cell plays an important role in diabetes. Whether XKP influences the viability of pancreatic β cells remains to be substantiated. In the present study, autophagy/apoptosis analyses were used to evaluate the therapeutic effect of XKP on pancreatic β-cells induced by high glucose levels and to investigate a potential causal molecular mechanism of XKP effect on the cells. The pancreatic β-cell lines MIN-6 were divided into four groups: control, high glucose (33.3 mmol/L), high glucose with XKP, high glucose with XKP and 3-Methyladenine (3-MA). Immunofluorescence assay was employed to determine autophagosome formation and flow cytometry was used to determine apoptotic rates of the β cells by the detecting expression of autophagy- and apoptosis-related proteins. High glucose increased the apoptotic rate of β-cells from 5.37% to 23.24%; however addition of XKP mitigated the rate at 10.92%. Data indicate that autophagy of β-cells was induced by XKP via the mammalian target of rapamycin (mTOR) pathway. Where the autophagy inhibitor 3-MA was added, the apoptotic rate was 23.94%, similar to the high glucose group rate. The results suggest a potential cytoprotective effect of XKP from high glucose toxicity by its induction of autophagy which may be linked to mTOR-mediated autophagy.

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