Theranostics with Hybrid Liposomes in an Orthotopic Graft Model Mice of Breast Cancer

Background/Aim: This study aimed to elucidate the therapeutic effects of hybrid liposomes (HL) composed of L-α-dimyristylphosphatidylcholine (DMPC) and polyoxyethylene [25] dodecyl ether (C₁₂(EO)₂₅) and the ability of HL-containing fluorescent probe to detect cancer in orthotopic graft model mice of breast cancer (MDA-MB-453). Materials and Methods: HL composed of 90 mol% DMPC and 10 mol% C₁₂(EO)₂₅ were prepared by the sonication method. Anti-tumor activities of HL were investigated in vivo using orthotopic graft-bearing mice of MDA-MB-453 cells. Results: With regard to the therapeutic effects of HL for breast cancer, HL inhibited the growth of MDA-MB-453 cells and induced apoptosis. Intravenous administration of HL resulted in a remarkable reduction of relative tumor weight in orthotopic graft model mice of breast cancer. The TUNEL assay revealed that this effect was due to induction of apoptosis. With regard to detection (diagnosis) of breast cancer, enhanced accumulation of HL carrying a fluorescence probe (Indocyanine green; ICG) was observed for MDA-MB-453 cells, although no accumulation of HL/ICG was obtained for normal breast cells. Enhanced accumulation of HL/ICG into the tumor of orthotopic graft model mice of breast cancer was observed. Conclusion: HL and HL/ICG could be theranostic targets since they showed therapeutic effects and ability to detect (diagnose) cancer in an orthotopic graft model mouse of breast cancer (MDA-MB-453).

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