Human Atrial Natriuretic Peptide in Cold Storage of Donation after Circulatory Death Rat Livers: An Old but New Agent for Protecting Vascular Endothelia?

Background Current critical shortage of donor organs has increased the use of donation after circulatory death (DCD) livers for transplantation, despite higher risk for primary non-function or ischemic cholangiopathy. Human atrial natriuretic peptide (hANP) is a cardiovascular hormone that possesses protective action to vascular endothelia. We aimed to clarify the therapeutic potential of hANP in cold storage of DCD livers. Methods Male Wistar rats were exposed to 30-minute warm ischemia in situ. Livers were then retrieved and cold-preserved for 6 hours with or without hANP supplementation. Functional and morphological integrity of the livers was evaluated by oxygenated ex vivo reperfusion at 37°C. Results hANP supplementation resulted in significant reduction of portal venous pressure (12.2 ± 0.5 vs. 22.5 ± 3.5 mmHg, P

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