Liver-type of tissue non-specific alkaline phosphatase is induced during mouse bone and tooth cell differentiation

Publication date: Available online 2 November 2018

Source: Archives of Oral Biology

Author(s): Tomomi T. Baba, Tatsuo Terashima, Shinichiro Oida

Abstract

Background and objective

Tissue non-specific alkaline phosphatase (TNSALP) contains two types—bone- and liver-type—which are produced from the same gene due to differences in splicing. These two differ in their promoter, but the amino acid sequences of the mature proteins are identical. In this study, we examined the relationship between the two types of TNSALP expression and osteoblast differentiation.

Design

Gene expression of the two types of TNSALP was observed by reverse transcription-polymerase chain reaction. MC3T3-NM4 was sub-cloned from an established mouse osteoblastic cell line in which osteoblast characters do not appear without dexamethasone. The C2C12 mouse myoblastic cell line, which can be induced to osteoblasts with bone morphogenic protein2, and organ-cultured tooth germs were also used in this work.

Results

The gene expression of liver-type TNSALP was observed in only MC3T3-NM4 activated by dexamethasone. For C2C12, the gene expression of bone-type TNSALP was observed even in non-induced conditions where myotubes were formed, whereas the liver-type TNSALP mRNA was only expressed when C2C12 differentiated into osteoblasts by bone morphogenic protein 2. Furthermore, in the organ-cultured tooth germs, the liver-type TNSALP mRNA was expressed according to differentiation of tooth germs.

Conclusion

These results suggest that the liver-type TNSALP mRNA is induced according to differentiation of bone and tooth.

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