Effect of Virus-Inactivating Agents on the Immunogenicity of Hantavirus Vaccines against Hemorrhagic Fever with Renal Syndrome

xlomafota.13 shared this article with you from Inoreader Effect of Virus-Inactivating Agents on the Immunogenicity of Hantavirus Vaccines against Hemorrhagic Fever with Renal Syndrome Via Latest Results for Applied Biochemistry and Microbiology Abstract Hemorrhagic fever with renal syndrome (HFRS) is an acute zoonotic disease caused by the orthohantaviruses Puumala, Dobrava-Belgrad (four genotypes), Hantaan (genotype Amur), and Seoul. In the Russian Federation, HFRS occupies the leading place among all natural focal human diseases. Formaldehyde, β-propiolactone, and ultraviolet radiation were tested to select the optimal method for viral inactivation during the development of a whole-virion vaccine against HFRS. The specific activity and the immunogenicity of the vaccine were determined, respectively, by the number of copies of viral RNA per 1 mL and by the neutralizing antibodies in the blood sera of BALB/c mice in response to immunization. Analysis of the immunogenicity of vaccines inactivated with formaldehyde, β-propiolactone, or UV radiation did not find significant differences in the level of induced neutralizing antibodies. At the same time, β-propiolactone has obvious technological advantages as c ompared to formaldehyde and UV radiation: the time of viral inactivation is reduced by dozens of times; neutralization of the inactivator and control of its content in the final vaccine are not required; and the amount of inappropriate protein in the vaccine is decreased due to the decrease in protein aggregation. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.