A high‐throughput screening platform for pigment regulating agents using pluripotent stem cell‐derived melanocytes

xlomafota13 shared this article with you from Inoreader A high‐throughput screening platform for pigment regulating agents using pluripotent stem cell‐derived melanocytes Via Experimental Dermatology Abstract In this study, we describe a simple and straightforward assay using induced pluripotent stem cell‐derived melanocytes and high‐throughput flow cytometry, to identify the effect induced by pigment regulating agents on melanin content. The assay is based on the correlation between forward light‐scatter characteristics and melanin content, with pigmented cells displaying high light absorption/low forward light scatter, while the opposite is true for lowly pigmented melanocytes, as a result of genetic background or chemical treatments. Orthogonal validation is then performed by regular melanin quantification. Such approach was validated using a set of 80 small molecules and yielded a confirmed hit. The assay described in this study may prove a useful tool to identify modulators of melanogenesis in human melanocytes. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.