Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells

xlomafota13 shared this article with you from Inoreader Piperazine ferulate prevents high-glucose-induced filtration barrier injury of glomerular endothelial cells Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1175. doi: 10.3892/etm.2021.10607. Epub 2021 Aug 13. ABSTRACT Filtration barrier injury induced by high glucose (HG) levels leads to the development of diabetic nephropathy. The endothelial glycocalyx plays a critical role in glomerular barrier function. In the present study, the effects of piperazine ferulate (PF) on HG-induced filtration barrier injury of glomerular endothelial cells (GEnCs) were investigated and the underlying mechanism was assessed. Immunofluorescence was used to observe the distribution of the glycocalyx as well as the expression levels of syndecan-1 and Zonula occludens-1 (ZO-1). Endothelial permeability assays were performed to assess the effects of PF on the integrity of the filtration barrier. Protein and mRNA expression levels were measured by western blotting and reverse transcription-quantitative PCR analyses, respectively. In vitro experiments revealed that adenosine mon ophosphate-activated protein kinase (AMPK) mediated HG-induced glycocalyx degradation and endothelial barrier injury. PF inhibited the HG-induced endothelial barrier injury and restored the expression levels of heparanase-1 (Hpa-1), ZO-1 and occludin-1 by AMPK. In vivo assays demonstrated that PF reduced the expression levels of Hpa-1, increased the expression levels of ZO-1 and attenuated glycocalyx degradation in the glomerulus. These data suggested that PF attenuated HG-induced filtration barrier injury of GEnC by regulating AMPK expression. PMID:34504620 | PMC:PMC8393711 | DOI:10.3892/etm.2021.10607 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.