A GntR Family Transcription Factor in Porphyromonas gingivalis Regulates Bacterial Growth, Acylpeptidyl Oligopeptidase and Gingipains Activity

alexandrossfakianakis shared this article with you from Inoreader A GntR Family Transcription Factor in Porphyromonas gingivalis Regulates Bacterial Growth, Acylpeptidyl Oligopeptidase and Gingipains Activity via Molecular Oral Microbiology SUMMARY Porphyromonas gingivalis (P. gingivalis) is a keystone pathogen for periodontitis. The function of the GntR family transcription factor is poorly studied in P. gingivalis. Numerous processes govern bacterial growth. The survival and pathogenicity of P. gingivalis depend heavily on its capacity to acquire amino acids as nutritional sources. In this investigation, a GntR transcription factor, pg1007, was identified in P. gingivalis, the deletion of which significantly inhibited bacterial growth. The mutant strain also exhibited increased extracellular activity of gingipains and acylpeptidyl oligopeptidase (AOP). Global gene expression profiling revealed that the expression levels of 59 genes were significantly altered in the Δpg1007 mutant, with an up-regulation in gene expression for AOP, ABC transporters, and some membrane proteins. In addition, His-PG1007 protein was purified as a recombinant protein from Escherichia coli, and the conserved DNA sequence bound by it was determined using electrophoretic mobility shift assays (EMSA) and DNase I footprinting assays. Consequently, this study demonstrated that pg1007 is a crucial transcription factor in P. gingivalis and regulates the bacterial growth and activity of gingipains and AOP. These findings may enhance our understanding of the regulation of bacterial proliferation and protease activity in P. gingivalis. This article is protected by copyright. All rights reserved View on Web

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