Cell-type and Tissue Context-dependent Nuclear Distribution of Human Ago2.

Cell-type and Tissue Context-dependent Nuclear Distribution of Human Ago2.

J Biol Chem. 2015 Dec 23;

Authors: Sharma NR, Wang X, Majerciak V, Ajiro M, Kruhlak M, Meyers C, Zheng ZM

Abstract
Argonaute-2 protein (Ago2), a major component of RISC, has been viewed as a cytoplasmic protein. In this report, we demonstrated by IFA confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic HPV16 or HPV18 infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell-type and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.

PMID: 26699195 [PubMed - as supplied by publisher]

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