Simultaneous detection of multiple lower genital tract pathogens by an impedimetric immunochip

Publication date: 15 May 2016
Source:Biosensors and Bioelectronics, Volume 79
Author(s): Maria Serena Chiriacò, Elisabetta Primiceri, Francesco De Feo, Alessandro Montanaro, Anna Grazia Monteduro, Andrea Tinelli, Marcella Megha, Davide Carati, Giuseppe Maruccio
Lower genital tract infections caused by both sexually and not-sexually transmitted pathogens in women are a key public health priority worldwide, especially in developing countries. Since standard analyses are time-consuming, appropriate therapeutic intervention is often neglected or delayed. Lab-on-chips and biosensors open new perspectives and offer innovative tools to simplify the diagnosis by medical staff, especially in countries with inadequate resources. Here we report a biosensing platform based on Electrochemical Impedance Spectroscopy (EIS) that allows multiplexed detection of Candida albicans, Streptococcus agalactiae and Chlamydia trachomatis with a single biochip, enabling a quick screening thanks to the presence of different immobilized antibodies, each specific for one of the different target pathogens.



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