LL37:DNA complexes provide antimicrobial activity against intracellular bacteria in human macrophages.

LL37:DNA complexes provide antimicrobial activity against intracellular bacteria in human macrophages.

Immunology. 2016 May 14;

Authors: Stephan A, Batinica M, Steiger J, Hartmann P, Zaucke F, Bloch W, Fabri M

Abstract
As part of the innate host response neutrophils release neutrophil extracellular traps (NETs), protein:DNA complexes that contain a number of antimicrobial peptides (AMPs), such as cathelicidin. Human cathelicidin in its active form, LL37, has potent antimicrobial activity against bacteria. However, whether LL37 derived from NETs contributes to antimicrobial activity against intracellular pathogens remains unclear. Here, we report that NETs induced by mycobacteria contain cathelicidin. Human macrophages internalized NET-bound cathelicidin, which is transported to lysosomal compartments. Furthermore, using a model of in vitro-generated LL37:DNA complexes we found that LL37 derived from such complexes attacks mycobacteria in macrophage phagolysosomes resulting in antimicrobial activity. Taken together, our results suggest a mechanism, by which LL37 in complex with DNA contributes to host defense against intracellular bacteria in human macrophages. This article is protected by copyright. All rights reserved.

PMID: 27177697 [PubMed - as supplied by publisher]

from Head and Neck on PubMed via xlomafota13 on Inoreader http://ift.tt/2529oXM
via IFTTT

from #Med Blogs by Alexandros G.Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1TeN9Dj
via IFTTT