Assay of superoxide dismutase activity in a plate assay using WST-1

Publication date: Available online 23 December 2016
Source:Free Radical Biology and Medicine
Author(s): Alexander V Peskin, Christine C Winterbourn
One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.

Graphical abstract

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