CD14++ CD16− monocytes are the main source of 11β-HSD type 1 after IL-4 stimulation

Publication date: February 2017
Source:International Immunopharmacology, Volume 43
Author(s): Vidya Kunnathully, Macarena Gomez-Lira, Giulio Bassi, Fabio Poli, Elisa Zoratti, Valentina La Verde, Luca Idolazzi, Davide Gatti, Ombretta Viapiana, Silvano Adami, Maurizio Rossini
The anti-inflammatory actions of IL-4 are well established through earlier findings. However, the exact mechanism it uses to downregulate the pro-inflammatory cytokine production through monocytes and macrophages is poorly understood. In this study, we examined the effect of IL-4 in the induction of 11β-HSD1 in the two main classes of monocytes, CD14⁺⁺ CD16⁻ (CD14) and CD14⁺ CD16⁺ (CD16). Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 17 healthy donors and were sorted into CD14 and CD16 subpopulations using cell sorting. Effect of IL-4 on 11β-HSD1-enzyme activity was measured in sorted and unsorted monocytes using Homogeneous Time-Resolved Fluorescence (HTRF) and M1/M2 polarization analysis was performed by flow cytometry. Our results indicate that CD14 cells are the major source of 11β-HSD1 enzyme after IL-4 stimulation and that M2 phenotype is not a pre-requisite for its synthesis.



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