Publication date: 15 May 2017
Source:Biosensors and Bioelectronics, Volume 91
Author(s): Xiaoke Yang, Xiaoming Zhou, Minjun Zhu, Da Xing
Pathogens pose a significant threat to public health worldwide. Despite many technological advances in the rapid diagnosis of pathogens, sensitive pathogen detection remains challenging because target pathogenic bacteria usually exist in complex samples at very low concentrations. Here, the construction of multivalent brush-like magnetic nanoprobes and their application for the efficient enriching of pathogens are demonstrated. Brush-like magnetic nanoprobes were constructed by modification with poly-L-lysine (PLL) onto amino-modified magnetic beads, followed by coupling of PEG (amine-PEG5000-COOH) to the amine sites of PLL. Subsequently, vancomycin (Van), a small-molecule antibiotic with affinity to the terminal peptide (D-alanyl-D-alanine) on the cell wall of Gram-positive bacteria, was conjugated to the carboxyl of the PEG. The use of multivalent brush-like magnetic nanoprobes (Van-PEG-PLL-MNPs) results in a high enrichment efficiency (>94%) and satisfactory purity for Listeria monocytogenes (employed as a model) within 20min, even at bacterial concentrations of only 102cfumL-1. Integrated with the enrichment of the Van-PEG-PLL-MNP nano-platform and electrochemiluminescence (ECL) detection, Listeria monocytogenes can be rapidly and accurately detected at levels as low as 10cfumL-1. The approach described herein holds great potential for realizing rapid and sensitive pathogen detection in clinical samples.
http://ift.tt/2ihZWNM
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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- Highly sensitive and low operating temperature SnO...
- Use of pyrolyzed paper as disposable substrates fo...
- Au Decorated ZnO hierarchical architectures: Facil...
- Effectiveness of the European Chromium VI Directiv...
- Ultrasonic-promoted rapid TLP bonding of fine-grai...
- Excitation energy transfer in ruthenium (II)-porph...
- Fabrication of biodegradable Zn-Al-Mg alloy: Mecha...
- Synthesis, spectroscopic, cyclic voltammetry prope...
- A rapid screening platform for catalyst discovery ...
- Mast cells are permissive for rhinovirus replicati...
- Selenite modulates the level of phenolics and nutr...
- Performance of Mn-Zn ferrite magnetic fluid in a p...
- Using Objective Structured Clinical Examinations t...
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- The diagnosis of silent myocardial ischemia. Motio...
- Is there a relationship between non-obstructive co...
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