An adult mouse thyroid side population cell line that exhibits enriched epithelial-to-mesenchymal transition.

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An adult mouse thyroid side population cell line that exhibits enriched epithelial-to-mesenchymal transition.

Thyroid. 2017 Jan 27;:

Authors: Murata T, Iwadate M, Takizawa Y, Miyakoshi M, Hayase S, Yang W, Cai Y, Yokoyama S, Nagashima K, Wakabayashi Y, Zhu J, Kimura S

Abstract
BACKGROUND: Studies of thyroid stem/progenitor cells have been hampered due to the small organ size and lack of tissue, which limits the yield of these cells. A continuous source that allows the study and characterization of thyroid stem/progenitor cells is desired to push the field forward.
METHOD: A cell line was established from Hoechst-resistant side population cells derived from mouse thyroid that were previously shown to contain stem/progenitor-like cells. Characterization of these cells were carried out by using in vitro 2- and 3-dimensional cultures and in vivo reconstitution of mice, in conjunction with qRT-PCR, western blotting, immunohisto(cyto)chemistry/immunofluorescence, and RNA-seq analysis.
RESULTS: These cells were named SPTL (side population cells-derived thyroid cell line). Under low serum culturing conditions, SPTL cells expressed the thyroid differentiation marker NKX2-1, a transcription factor critical for thyroid differentiation and function, while no expression of other thyroid differentiation marker genes were observed. SPTL cells formed follicle-like structures in matrigel cultures, which did not express thyroid differentiation marker genes. In mouse models of orthotopic and intravenous injection, the latter following partial thyroidectomy, a few SPTL cells were found in part of the follicles, most of which expressed NKX2-1. SPTL cells highly express genes involved in epithelial-mesenchymal transition as demonstrated by RNA-seq analysis, and exhibit a gene expression pattern similar to anaplastic thyroid carcinoma.
CONCLUSION: These results demonstrate that SPTL cells have the capacity to differentiate into thyroid to a limited degree. SPTL cells may provide an excellent tool to study stem cells, including cancer stem cells of the thyroid.

PMID: 28125936 [PubMed - as supplied by publisher]

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