Abstract
Objective
Halitosis is caused by volatile sulfur compounds including methyl mercaptan (CH3SH) in the oral cavity and is a serious problem that limits interpersonal social communication. The aim of study was to evalute the effects of reuterin-related compounds (RRCs) on halitosis-related periodontopathic bacteria in vitro.
Materials And Methods
RRC-01, -02 and -03 (32 and 64 μg/mL) in culture media containing Fusobacterium nucleatum JCM8523 and Porphyromonas gingivalis ATCC33277 were used. The effects of RRCs on CH3SH production and detectable odour by F. nucleatum and P. gingivalis were examined by CH3SH production assay and organoleptic test, respectively. The number of bacterial cells was also measured using an ATP assay. In P. gingivalis treated with RRCs, the expression of mgl gene, which is responsible for CH3SH production, was examined by qRT-PCR.
Results
CH3SH production and the score of detectable odour from F. nucleatum and P. gingivalis culture media containing RRCs were significantly lower than that without RRCs (p< 0.05). The expression of mgl gene in P. gingivalis was significantly down-regulated by RRC-01 (p< 0.01), but not by RRC-02 or -03.
Conclusions
RRCs are potent oral care products for preventing halitosis via reducing CH3SH production.
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