DNA methylation regulates gene expression in intracranial aneurysms.

DNA methylation regulates gene expression in intracranial aneurysms.

World Neurosurg. 2017 Apr 19;:

Authors: Yu L, Wang J, Wang S, Zhang D, Zhao Y, Wang R, Zhao J

Abstract
OBJECTIVE: Different gene expression profiles are observed in intracranial aneurysm tissue. Understanding these genes and what regulates their expression will help us understand intracranial aneurysm pathogenesis. We investigated whether differences in DNA methylation regulate gene expression in intracranial aneurysms.
METHODS: Twenty intracranial aneurysm tissues were compared with 20 matched samples from the superficial temporal artery as controls. We detected the gene expression profiles in these samples using Human U133 Plus 2.0 Gene Chip and the levels of DNA methylation modification using Infinium Human Methylation 450 Bead Chip.
RESULTS: A total of 11,022 differentially methylated sites between aneurysm tissues and matched control tissues were identified. Moreover, 2,142 differentially expressed gene transcripts were detected based on the two gene expression profile. GO and KEGG analysis and verification analysis showed that the MYH11, LIFR, and TLR4 genes were associated with the occurrence and development of intracranial aneurysm. These genes mainly encode cell adhesion molecules (CAMs), or are involved in the NF-κB, JAK-STAT, and ERK/JNK signaling pathways.
CONCLUSION: In the development of intracranial aneurysms, DNA methylation plays an important role in the genetic expression regulation involved in immune and inflammatory reactions, cell function, cell maintenance, and cell signal transduction.

PMID: 28433851 [PubMed - as supplied by publisher]

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