Synthesis and characterization of a bimodal nanoparticle based on the host-guest self-assembly for targeted cellular imaging

Publication date: 15 August 2017
Source:Talanta, Volume 171
Author(s): Huihui Wang, Dongsheng Sun, Han Liao, Yanfang Wang, Shan Zhao, Ying Zhang, Guojun Lv, Xiaojun Ma, Yang Liu, Guangwei Sun
Multimodal imaging provides distinct advantages over traditional single modal imaging. The combined modalities of magnetic resonance imaging (MRI) and near-infrared imaging (NIR), in particular, provide a powerful tool for tumor diagnosis. In this study, a bimodal MRI and NIR self-assembled supramolecular nanoparticle was developed via the self-assembly of host-guest interactions between hyaluronic acid–β-cyclodextrin (HA–CD) and amantadine (Ad)-modified imaging agents (Gd–DOTA and NIR cyanine dye Cy7). The supramolecular HA–CD–GC nanoparticles (NPs) were characterized by transmission electron microscopy (TEM), Zeta potential, and dynamic light-scattering (DLS) experiments. The relaxivity and fluorescent properties of the NPs were also determined. HA–CD–GC NPs exhibited an enhanced relaxivity of 11.4mM⁻¹S⁻¹, which was three-fold higher than that of clinical Gd³⁺-chelated complex, for MRI imaging. Moreover, HA–CD–GC NPs displayed excellent fluorescence. In addition, HA–CD–GC NPs were internalized into tumor cells via HA-receptor CD44-mediated endocytosis. Therefore, the self-assembled HA–CD–GC NPs are effective targeted tumor cell imaging systems and have potential applications in cancer diagnosis and treatment.

Graphical abstract

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