Y-box Binding Protein 1 Is Involved in Regulating the G2/M Phase of the Cell Cycle

Background/Aim: The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G₁ phase of the cell cycle by controlling transcription of G₁ regulators. Here, we report that YB1 is also involved in regulating G₂/M phase. Materials and Methods: YB1-depleted TKO cells were subjected to quantitative reverse transcription-polymerase chain reaction and cell-cycle analysis. RNA immunoprecipitation (RIP)-chip assay was performed using anti-YB1 antibodies. Precipitated RNAs were subjected to microarray analysis. Results: Silencing YB1 inhibited the proliferation of TKO cells, which lost the machinery required for G₁ phase arrest. Cell-cycle analysis showed that silencing YB1 caused G₂/M phase cell-cycle arrest. RIP-chip assay showed that YB1 associated with mRNA of multiple cell-cycle-related genes, including G₂/M phase regulators. Conclusion: YB1 positively regulates not only the G₁ phase but also G₂/M phase by regulating multiple cell-cycle-related genes.

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