Reduced MAD2 levels dampen apoptotic response to non-exchange sex chromosomes and lead to sperm aneuploidy [RESEARCH ARTICLE]

Imrul Faisal and Liisa Kauppi

In meiosis, non-exchange homologous chromosomes are at risk for missegregation and should be monitored by the spindle assembly checkpoint (SAC) to avoid formation of aneuploid gametes. Sex chromosome missegregation is particularly common and can lead to sterility or to aneuploid offspring (e.g. individuals with Turner or Klinefelter syndrome). Despite major implications for health and reproduction, modifiers of meiotic SAC robustness and the subsequent apoptotic response in male mammals remain obscure. Levels of SAC proteins, e.g. MAD2, are critical for normal checkpoint function in many experimental systems, but surprisingly, apparently not in male meiosis, as indicated by the lack of chromosome segregation defects reported earlier in Mad2^+/- spermatocytes. To directly test whether MAD2 levels impact the meiotic response to missegregating chromosomes, we utilized Spo11β-only^mb mice that are prone to non-exchange X-Y chromosomes. We show that reduced MAD2 levels attenuate the apoptotic response to missegregating sex chromosomes and allow the formation of aneuploid sperm. These findings demonstrate that SAC protein levels are critical for the efficient elimination of aberrant spermatocytes.

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