Anti-proliferative activity of Gum kondagogu (Cochlospermum gossypium)-gold nanoparticle constructs on B16F10 melanoma cells: an in vitro model

Publication date: Available online 11 July 2017
Source:Bioactive Carbohydrates and Dietary Fibre
Author(s): S. Kalaignana Selvi, M.J. Mahesh Kumar, R.B. Sashidhar
The present study has explored the reducing and capping potentials of gum kondagogu (GK) for the synthesis of gold nanoparticle constructs. The formation of gold nanoparticles was confirmed by UV- absorption, FTIR, AFM, SEM and TEM. The GK-AuNPs showed a characteristic absorption peak at 534nm in UV spectra with the size range of 4.08–12.73 ±0.75nm. As a result of wide screening on the application of newly synthesized GK-AuNPs their anticancer potential were discovered by MTT assay, confocal microscopy, flow cytometry and quantitative real time PCR techniques. MTT assay of synthesized GK-AuNPs exhibited IC50 concentration of 1.7µgL⁻¹ after 48h. FACS analysis revealed that cell cycle gets arrested at G1 to S phase, with up-regulation of p53, caspase-3, caspase-9, PPARa and PPARb, genes and down regulation of Bcl-2 and Bcl-x(K) genes, in B16F10 cells treated with GK-AuNPs confirming the anti-proliferative properties of the nanoparticles.

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