Probing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping

Publication date: 11 July 2017
Source:Cell Reports, Volume 20, Issue 2
Author(s): Giulia Varsano, Yuedi Wang, Min Wu
Cell size homeostasis can be achieved by size checkpoints that couple cell size to cell-cycle progression or by alternative mechanisms such as constant extension. In mammalian cells, the existence of strict size checkpoints remains controversial due to the technical limitations in determining cell size directly and accurately. We developed a microfabricated channel system that linearizes mammalian cell growth and facilitates cell size measurements. By tracking cell length, while directly visualizing cell-cycle progression in rat basophilic leukemia cells and RAW 264.7 macrophages, we examined the mechanisms of size homeostasis and the existence of a size checkpoint at the G1/S transition. Our analysis revealed a two-tier size homeostasis mechanism where a G1 "sizer" or "adder" could operate, depending on the birth size of the cells.

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Teaser

Varsano et al. have developed a direct approach for measuring cell size changes of individual growing mammalian cells in relation with cell-cycle progression. They discovered that mammalian cells could use either a G1/S size checkpoint or constant extension for size control, depending on their birth sizes.


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