Publication date: November 2017
Source:The Journal of Steroid Biochemistry and Molecular Biology, Volume 174
Author(s): Valentin Trujillo, Paulina Marín-Luevano, Irma González-Curiel, Adrián Rodríguez-Carlos, Maira Ramírez-Reyes, Esther Layseca-Espinosa, José A. Enciso-Moreno, Lorenza Díaz, Bruno Rivas-Santiago
Foot ulceration is one of the most common and complex sequelae of diabetes mellitus, generally posing a therapeutic challenge due to poor healing responses and high rates of complications, including peripheral vascular disease, ischemia and infections. Calcitriol, the most active vitamin D metabolite, induces antimicrobial peptides production in keratinocytes from diabetic foot ulcers (DFU); however, little is known about its effects on angiogenic factors in this pathology. Herein we aimed at studying whether calcitriol induces angiogenic molecules in keratinocytes under normoxic and hypoxic conditions, and if these molecules are able to improve cell migration in vitro. Evaluation of DFU samples by immunohistochemistry showed increased VEGF and decreased angiogenin and HIF-1α expression compared to controls, suggesting an altered pattern of angiogenic factors in DFU. Interestingly, incubation of keratinocytes with calcitriol significantly upregulated VEGFA, HIF-1α and angiogenin gene expression, while the resulting cell culture media stimulated both endothelial cells and keratinocytes migration in an in vitro wound closure assay under a normoxic environment (p<0.05). Moreover, the culture media of calcitriol-treated keratinocytes stimulated cell migration in a similar extent as exogenous VEGF or EGF in endothelial and keratinocytes cells. These results suggest that the altered profile of angiogenic molecules in DFU might be improved by local or systemic treatment with calcitriol under normoxic conditions, which could probably be achieved with hyperbaric oxygen therapy. Given that calcitriol not only augments proangiogenic factors but also induces antimicrobial peptides expression, this hormone should be further investigated in clinical trials of DFU.
http://ift.tt/2iya42E
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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Δευτέρα 6 Νοεμβρίου 2017
Calcitriol promotes proangiogenic molecules in keratinocytes in a diabetic foot ulcer model
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- Developmental refinement of synaptic transmission ...
- Photoluminescent properties of single crystal diam...
- Does the reduction of inferior turbinate size affe...
- Pharmacotherapy for social anxiety disorder: Inter...
- A case of segmental stiff skin syndrome treated wi...
- CONTENTS 1
- CONTENTS 2
- Intralabyrinthine sporadic endolymphatic sac tumour
- Direct Observation vs. Video-Based Assessment in F...
- Characterization of Vessel Deformations During EVA...
- Analysis of Academic Medical Center Graduate Medic...
- 360° Operative Videos: A Randomised Cross-Over Stu...
- Textual Analysis of General Surgery Residency Pers...
- Central leptin regulates heart lipid content by se...
- Vitamin D, the placenta and early pregnancy: effec...
- Visible-light-driven photocatalytic inactivation o...
- Encapsulation of fluorescein into nanozeolites L a...
- Utilization of red mud and Pb/Zn smelter waste for...
- A novel method of utilization of hot dip galvanizi...
- A detailed study on Sn4+ doped ZnO for enhanced ph...
- Zn nanoparticle formation in FIB irradiated single...
- Effects of the carrier concentration on polarity d...
- Evaluating the diffusive gradients in thin films t...
- Soil geochemical factors regulate Cd accumulation ...
- Study of the iron nitride FeN into the megabar regime
- Public health approach to minimize the prevalence ...
- Necessity to strengthen prevention activities and ...
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- Building an effective mechanism to respond to the ...
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- Percutaneous CT-guided facet joint synovial cyst r...
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- Value of elective radiation oncology rotations: ho...
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- Sleep Apnea and Cardiovascular Disease: Lessons Fr...
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- An Asymptomatic Man With an Abnormal ECG.
- Impact of Heart Outcomes Prevention Evaluation Tri...
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- Letter by Maltais et al Regarding Article, "Hemoco...
- Response by Mehra et al to Letter Regarding Articl...
- Update to Practice Standards for Electrocardiograp...
- Correction to: Role of Biomarkers for the Preventi...
- Correction to: American Heart Association's Call t...
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- Self-assembling peptide hydrogel enables instant e...
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- Heme and hemolysis in innate immunity: adding insu...
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- Single-Neuron Correlates of Awareness during Atten...
- Parallel Distributed Processing Theory in the Age ...
- Network Design and the Brain
- Visual Working Memory Storage Recruits Sensory Pro...
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- Phytochemical profile and mechanisms involved in t...
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- Hepatoprotective effect of berberine against metho...
- Curcumin and endometriosis: Review on potential ro...
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- The role of SIRT1 in diabetic retinopathy
- Taurine enhances spermatogenic function and antiox...
- CTRP6 inhibits cell proliferation and ECM expressi...
- The relevance of folkloric usage of plant galls as...
- Cytotherapy using stromal cells: Current and advan...
- Pharmacological effects and potential therapeutic ...
- Subthreshold diode micropulse laser versus convent...
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