Microarray analysis of lncRNA and mRNA expression profiles in mice with allergic rhinitis

Publication date: January 2018
Source:International Journal of Pediatric Otorhinolaryngology, Volume 104
Author(s): Yue Ma, Le Shi, Chunquan Zheng
ObjectivesWe aimed to identify the effect of lncRNAs in CD4⁺ T cells on Allergic rhinitis (AR).MethodsThe present study conducted a microarray to identify the expression profiles of lncRNA and mRNA in CD4⁺ T cells in both AR murine models and normal controls. And qRT-PCR was used to confirm the results. GO and KEGG enrichment analysis were used to show all related pathways and a co-expression network was conducted to find lncRNAs which have high correlation with these pathways.ResultsThe results showed that the two groups contained a total of 158 deregulated lncRNAs, of which 110 were upregulated and 48 were downregulated. And positive regulation of calcium ion transport, B cell activation, chemokine-signaling pathways and calcium-signaling pathways may be involved in the development of T cells in AR pathology. Finally, we can find the differentially expressed mRNA in the pathways related to T cell differentiation correlated with many deregulated lncRNAs.ConclusionsThe present study was the first to show the differential expression profiles of lncRNAs in the CD4⁺ T cells of an AR murine model, which may provide significant insights into AR pathogenesis and offer new treatment targets to alleviate it.



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