Publication date: February 2018
Source:Biomaterials, Volume 155
Author(s): Zongmin Zhao, Brian Harris, Yun Hu, Theresa Harmon, Paul R. Pentel, Marion Ehrich, Chenming Zhang
Current clinically-tested nicotine vaccines have yet shown enhanced smoking cessation efficacy due to their low immunogenicity. Achieving a sufficiently high immunogenicity is a necessity for establishing a clinically-viable nicotine vaccine. This study aims to facilitate the immunogenicity of a hybrid nanoparticle-based nicotine vaccine by rationally incorporating toll-like receptor (TLR)-based adjuvants, including monophosphoryl lipid A (MPLA), Resiquimod (R848), CpG oligodeoxynucleotide 1826 (CpG ODN 1826), and their combinations. The nanoparticle-delivered model adjuvant was found to be taken up more efficiently by dendritic cells than the free counterpart. Nanovaccine particles were transported to endosomal compartments upon cellular internalization. The incorporation of single or dual TLR adjuvants not only considerably increased total anti-nicotine IgG titers but also significantly affected IgG subtype distribution in mice. Particularly, the nanovaccines carrying MPLA+R848 or MPLA+ODN 1826 generated a much higher anti-nicotine antibody titer than those carrying none or one adjuvant. Meanwhile, the anti-nicotine antibody elicited by the nanovaccine adjuvanted with MPLA+R848 had a significantly higher affinity than that elicited by the nanovaccine carrying MPLA+ODN 1826. Moreover, the incorporation of all the selected TLR adjuvants (except MPLA) reduced the brain nicotine levels in mice after nicotine challenge. Particularly, the nanovaccine with MPLA+R848 exhibited the best ability to reduce the level of nicotine entering the brain. Collectively, rational incorporation of TLR adjuvants could enhance the immunological efficacy of the hybrid nanoparticle-based nicotine vaccine, making it a promising next-generation immunotherapeutic candidate for treating nicotine addiction.
http://ift.tt/2A7rLOT
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
-
▼
Νοεμβρίου
(7424)
-
▼
Νοε 24
(154)
- A randomised phase II study of chemoradiotherapy w...
- Farewell to monomodality treatment in patients wit...
- The effectiveness of topical colloidal silver in r...
- The effectiveness of topical colloidal silver in r...
- Drug-excipient compatibility assessment of solid f...
- Dual-route targeted vaccine protects efficiently a...
- Nasopharyngeal s. pneumoniae carriage and density ...
- Will we have new pertussis vaccines?
- The efficacy of recombinant turkey herpesvirus vac...
- Experimental assessment of the Advanced Collapsed-...
- Dosimetric impact of inter-observer catheter recon...
- Biochar decreased the bioavailability of Zn to ric...
- Effects of dam construction and increasing polluta...
- Facile approach to synthesize highly fluorescent m...
- Nanoparticle-loaded Hydrogels as a Pathway for Enz...
- Infectious Complications following Breast Reconstr...
- Author Index
- Subject Index
- Instructions for Contributors
- TOC
- Calendar
- Editorial Board
- Calendar Listings
- Do Residency Selection Factors Predict Radiology R...
- Diagnostic Utility of 3T Lung MRI in Children with...
- Clinical and genetic characteristics of xeroderma ...
- Non-melanoma Hutchinson's sign: A reappraisal of t...
- In vivo Confocal Laser Microscopy for monitoring o...
- Novel device-based acne treatments: comparison of ...
- Educational and Practice Gaps in the Management of...
- Cutaneous granuloma caused by Rhizopus oryzae with...
- Response to – Bullous pemphigoid inguinalis?
- Bullous Pemphigoid Inguinalis?
- Electrochemotherapy of unresectable cutaneous tumo...
- Does obesity cause a distinct phenotype of hidrade...
- Association Between Pepsin in the Saliva and the S...
- A case of large cell neuroendocrine carcinoma of t...
- Cytokeratin 20/p53 dual immunocytochemistry for im...
- Dysregulatory effects of retinoic acid isomers in ...
- Volatile organic compounds and nitric oxide as res...
- Enhanced phosphorus removal using acid-treated mag...
- PCDD/PCDF formation in the chlor-alkali process—la...
- Dimensions of hard and soft tissue around adjacent...
- Recurrence of oral mucocoeles in adolescents after...
- Upregulation of angiogenesis in oral lichen planus
- Clinical effects of submucosal middle turbinectomy...
- Clinical effects of submucosal middle turbinectomy...
- The association between atopic dermatitis and hand...
- The association between atopic dermatitis and hand...
- Evidence for lattice softening of the Fe-Ga magnet...
- Compositional variation effects on the microstruct...
- Ex-vivo flush of the limb allograft reduces inflam...
- Erratum to “Reconstruction design before tumour re...
- Dual loss of p110δ PI3-kinase and SKAP (KNSTRN) ex...
- Sensitivity and specificity of Frontal Assessment ...
- Evidence-based and consensus clinical practice gui...
- Validation study of the Vitiligo Extent Score-plus...
- Estimating the cost of skin cancer detection by de...
- Future Considerations for Clinical Dermatology in ...
- Lack of correlation of the patient derived vitilig...
- Drug survival of secukinumab in real-world plaque ...
- Differentiating Merkel cell carcinoma of lymph nod...
- Initial brain aging: heterogeneity of mitochondria...
- NEK1 genetic variability in a Belgian cohort of AL...
- Thinner cortex in patients with subjective cogniti...
- Contents continued
- Splenectomy protects aged mice from injury after e...
- Editorial Advisory Board
- Neuronal and behavioral effects of multi-day brain...
- Contents
- Harmonization of cortical thickness measurements a...
- Characterization of epitope specificities of refer...
- Characterization of epitope specificities of refer...
- Time and motion studies of National Health Service...
- Macular thickness profile and diabetic retinopathy...
- Dermoscopy of different stages of lymphomatoid pap...
- All you should know about sunken eyes
- All you should know about sunken eyes
- A procedure to evaluate the factors determining th...
- Evaluation of sources and fate of nitrates in the ...
- Risk of inferior alveolar nerve injury with corone...
- Fast trace determination of nine odorant and estro...
- Study on diesel vertical migration characteristics...
- DIAGNOSIS OF ENDOCRINE DISEASE: 18-Oxocortisol and...
- Identifying and treating subclinical thyroid dysfu...
- European Society of Endocrinology Clinical Practic...
- Modulation of the gut microbiome: a systematic rev...
- DIAGNOSIS of ENDOCRINE DISEASE: SDHx mutations: be...
- Metformin in gestational diabetes mellitus: predic...
- Individual testosterone decline and future mortali...
- Matching-Adjusted Indirect Comparison of Efficacy ...
- Matching-Adjusted Indirect Comparison of Efficacy ...
- AHNS Series: Do you know your guidelines? Principl...
- Concomitant sensitization to legumin, Fag e 2 and ...
- Concomitant sensitization to legumin, Fag e 2 and ...
- AHNS Series: Do you know your guidelines? Principl...
- Meta-analysis of induction chemotherapy as selecti...
- IMRT vs 2DRT for NPC Patients
- Efficacy Of Clinical Pilates Exercises İn Desk-Bas...
- IMRT vs 2DRT for NPC Patients
-
▼
Νοε 24
(154)
- ► Σεπτεμβρίου (7549)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
►
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
▼
2017
(111579)
- ► Δεκεμβρίου (7718)
-
▼
Νοεμβρίου
(7424)
-
▼
Νοε 24
(154)
- A randomised phase II study of chemoradiotherapy w...
- Farewell to monomodality treatment in patients wit...
- The effectiveness of topical colloidal silver in r...
- The effectiveness of topical colloidal silver in r...
- Drug-excipient compatibility assessment of solid f...
- Dual-route targeted vaccine protects efficiently a...
- Nasopharyngeal s. pneumoniae carriage and density ...
- Will we have new pertussis vaccines?
- The efficacy of recombinant turkey herpesvirus vac...
- Experimental assessment of the Advanced Collapsed-...
- Dosimetric impact of inter-observer catheter recon...
- Biochar decreased the bioavailability of Zn to ric...
- Effects of dam construction and increasing polluta...
- Facile approach to synthesize highly fluorescent m...
- Nanoparticle-loaded Hydrogels as a Pathway for Enz...
- Infectious Complications following Breast Reconstr...
- Author Index
- Subject Index
- Instructions for Contributors
- TOC
- Calendar
- Editorial Board
- Calendar Listings
- Do Residency Selection Factors Predict Radiology R...
- Diagnostic Utility of 3T Lung MRI in Children with...
- Clinical and genetic characteristics of xeroderma ...
- Non-melanoma Hutchinson's sign: A reappraisal of t...
- In vivo Confocal Laser Microscopy for monitoring o...
- Novel device-based acne treatments: comparison of ...
- Educational and Practice Gaps in the Management of...
- Cutaneous granuloma caused by Rhizopus oryzae with...
- Response to – Bullous pemphigoid inguinalis?
- Bullous Pemphigoid Inguinalis?
- Electrochemotherapy of unresectable cutaneous tumo...
- Does obesity cause a distinct phenotype of hidrade...
- Association Between Pepsin in the Saliva and the S...
- A case of large cell neuroendocrine carcinoma of t...
- Cytokeratin 20/p53 dual immunocytochemistry for im...
- Dysregulatory effects of retinoic acid isomers in ...
- Volatile organic compounds and nitric oxide as res...
- Enhanced phosphorus removal using acid-treated mag...
- PCDD/PCDF formation in the chlor-alkali process—la...
- Dimensions of hard and soft tissue around adjacent...
- Recurrence of oral mucocoeles in adolescents after...
- Upregulation of angiogenesis in oral lichen planus
- Clinical effects of submucosal middle turbinectomy...
- Clinical effects of submucosal middle turbinectomy...
- The association between atopic dermatitis and hand...
- The association between atopic dermatitis and hand...
- Evidence for lattice softening of the Fe-Ga magnet...
- Compositional variation effects on the microstruct...
- Ex-vivo flush of the limb allograft reduces inflam...
- Erratum to “Reconstruction design before tumour re...
- Dual loss of p110δ PI3-kinase and SKAP (KNSTRN) ex...
- Sensitivity and specificity of Frontal Assessment ...
- Evidence-based and consensus clinical practice gui...
- Validation study of the Vitiligo Extent Score-plus...
- Estimating the cost of skin cancer detection by de...
- Future Considerations for Clinical Dermatology in ...
- Lack of correlation of the patient derived vitilig...
- Drug survival of secukinumab in real-world plaque ...
- Differentiating Merkel cell carcinoma of lymph nod...
- Initial brain aging: heterogeneity of mitochondria...
- NEK1 genetic variability in a Belgian cohort of AL...
- Thinner cortex in patients with subjective cogniti...
- Contents continued
- Splenectomy protects aged mice from injury after e...
- Editorial Advisory Board
- Neuronal and behavioral effects of multi-day brain...
- Contents
- Harmonization of cortical thickness measurements a...
- Characterization of epitope specificities of refer...
- Characterization of epitope specificities of refer...
- Time and motion studies of National Health Service...
- Macular thickness profile and diabetic retinopathy...
- Dermoscopy of different stages of lymphomatoid pap...
- All you should know about sunken eyes
- All you should know about sunken eyes
- A procedure to evaluate the factors determining th...
- Evaluation of sources and fate of nitrates in the ...
- Risk of inferior alveolar nerve injury with corone...
- Fast trace determination of nine odorant and estro...
- Study on diesel vertical migration characteristics...
- DIAGNOSIS OF ENDOCRINE DISEASE: 18-Oxocortisol and...
- Identifying and treating subclinical thyroid dysfu...
- European Society of Endocrinology Clinical Practic...
- Modulation of the gut microbiome: a systematic rev...
- DIAGNOSIS of ENDOCRINE DISEASE: SDHx mutations: be...
- Metformin in gestational diabetes mellitus: predic...
- Individual testosterone decline and future mortali...
- Matching-Adjusted Indirect Comparison of Efficacy ...
- Matching-Adjusted Indirect Comparison of Efficacy ...
- AHNS Series: Do you know your guidelines? Principl...
- Concomitant sensitization to legumin, Fag e 2 and ...
- Concomitant sensitization to legumin, Fag e 2 and ...
- AHNS Series: Do you know your guidelines? Principl...
- Meta-analysis of induction chemotherapy as selecti...
- IMRT vs 2DRT for NPC Patients
- Efficacy Of Clinical Pilates Exercises İn Desk-Bas...
- IMRT vs 2DRT for NPC Patients
-
▼
Νοε 24
(154)
- ► Σεπτεμβρίου (7549)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου