Three-dimensional and non-destructive characterization of nerves inside conduits using laboratory-based micro computed tomography

Publication date: Available online 10 November 2017
Source:Journal of Neuroscience Methods
Author(s): Christos Bikis, Peter Thalmann, Lucas Degrugillier, Georg Schulz, Bert Müller, Daniel F. Kalbermatten, Srinivas Madduri, Simone E. Hieber
BackgroundHistological assessment of peripheral nerve regeneration in animals is tedious, time-consuming and challenging for three-dimensional analysis.New MethodThe present study reports on how and to what extent micro computed tomography of paraffin-embedded samples can provide a reliable three-dimensional approach for quantitative analysis of peripheral nerves.ResultsRat sciatic nerves were harvested, formalin-fixated, positioned into nerve conduits (NC), paraffin-embedded, and imaged using a laboratory-based X-ray microtomography system with an isotropic voxel length of 4 μm. Suitable quantitative measures were identified and automatically evaluated, i.e. nerve length, cross-sectional area and volume, as well as vascular structures, to be used as an assessment and comparison indicator of regeneration quality.Comparison with Existing MethodsCompared to imaging using contrast agents, the investigated specimens can subsequently undergo the conventional histological analysis without requiring additional preparation steps. Contrast and spatial resolution are also increased significantly.ConclusionsWe demonstrate the potential of the micro computed tomography for non-destructive monitoring of peripheral nerves inside the conduits.



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