16S rRNA profiling of the Dermatophagoides farinae core microbiome: Enterococcus and Bartonella

Abstract

Characterization of the house dust mite (HDM; Dermatophagoides farinae) microbiome is important because it may exert immunomodulatory effects on the pathogenesis of allergic diseases, owing to its ability to generate microbe-associated molecules such as lipopolysaccharides and lipoteichoic acid.[1] In a recent study, whole-genome shotgun sequencing of D. farinae revealed that Enterobacter were the most abundant bacteria[2], while another study using 16S rRNA cloning reported that Bartonella was the most abundant bacterial taxon.[3] In this study, a 16S rRNA amplicon analysis using high-throughput sequencing technology was performed; this is currently the most widely used and reliable method for microbiome community studies.

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