Increased expression of Apo-J and Omi/HtrA2 after Intracerebral Hemorrage in rats.

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Increased expression of Apo-J and Omi/HtrA2 after Intracerebral Hemorrage in rats.

World Neurosurg. 2018 Mar 23;:

Authors: Li F, Yang J, Guo X, Zheng X, Lv Z, Shi CQ, Li X

Abstract
OBJECTIVE: To investigate the changes of Apo-J and Omi/HtrA2 protein expression in rats with intracerebral hemorrage.
METHODS: 150 SD adult rats were randomly divided into 3 groups: (1) Normal Control (NC) group, (2) Sham group, (3) Intracerebral Hemorrage (ICH) group. The data were collected at 6h, 12h, 1d, 2d, 3d, 5d and 7d. Apoptosis was measured by Tunel staining. The distributions of the Apo-J and Omi/HtrA2 proteins were determined by immunohistochemical staining. The levels of Apo-J mRNA and Omi/HtrA2 mRNA expressions were examined by RT-PCR.
RESULTS: Apoptosis in ICH group was higher than Sham and NC groups (p＜0.05). Both the Apo-J and Omi/HtrA2 expression levels were increased in the peripheral region of hemorrhage, with a peak at 3d. The Apo-J mRNA level positively correlated with HtrA2 mRNA level in ICH group (r=0.883, p＜0.001).
CONCLUSION: The expressions of Apo-J and Omi/HtrA2 paralelly increased in peripheral region of rat cerebral hemorrhage. Local high expressed Apo-J in the peripheral regions might play a neuroprotective role by inhibiting apoptosis via Omi/HtrA2 pathway after hemorrhage.

PMID: 29581019 [PubMed - as supplied by publisher]

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