Highly sensitive label-free amperometric immunoassay of prostate specific antigen using hollow dendritic AuPtAg alloyed nanocrystals

Publication date: 15 July 2018
Source:Biosensors and Bioelectronics, Volume 111
Author(s): Ya-Cheng Shi, Ai-Jun Wang, Pei-Xin Yuan, Lu Zhang, Xiliang Luo, Jiu-Ju Feng
Herein, well-defined hollow dendritic AuPtAg alloyed nanocrystals (ANCs) were synthesized by a simple L-proline-mediated one-pot aqueous method. More importantly, the synthesized hollow dendritic architectures provide a suitable platform for immobilization of anti-prostate specific antigen (PSA). The resultant label-free immunosensor exhibited the improved performance for highly sensitive detection of PSA based on the enhanced catalytic currents of K3[Fe(CN)6] as a signal probe. Impressively, the immunosensor showed the wide linear range of 0.05–50 ng mL^–1 and low detection limit of 0.017 ng mL^–1 under optimal conditions for the assay of PSA, couple with the improved stability, reproducibility and selectivity. It provides a promising platform for clinical research and diagnosis.



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