Publication date: October 2018
Source:Biomedicine & Pharmacotherapy, Volume 106
Author(s): Wenzhe Song, Parth Thakor, David A. Vesey, Glenda C. Gobe, Christudas Morais
Disparate roles exist for tumor-associated macrophages in breast cancer growth and progression. The aim of this study was to explore the influence of induced macrophages on the growth of breast cancer cells. THP-1 monocytes were differentiated to macrophages using phorbol 12-myristate 13-acetate. The effect of the medium from THP-1 monocytes or macrophage-conditioned medium (MφCM) on MCF-7 (estrogen receptor and progesterone-positive positive) and MDA-MB-231 (MB; triple-negative) breast cancer cells was determined at 24 h, 48 h and 72 h. Assays were conducted for cell viability, apoptosis, proliferation and cell phenotype, and quantitative real-time polymerase chain reaction (qRT-PCR) for expression of associated genes. MφCM inhibited proliferation of MCF-7 and MB cells in a time-dependent manner and, in particular, decreased viability of MCF-7 cells. MφCM induced a markedly vacuolated phenotype in MCF-7 increased apoptosis in MCF-7 cells, but correlative changes in Bcl-2 or Bax were absent. A multifold and significant reduction in anti-apoptotic Bcl-2 in MB cells was not matched by increased apoptosis. The cell cycle inhibitor CDKN1A was increased in both cell lines, but PCNA decreased only in MB cells. Senescence-associated galactosidase beta-1 (GLB1) mRNA was decreased in MCF-7 cells (48 and 72 h) but increased in MB cells (72 h). Increased expression of interleukin-6 (IL-6) and IL-8 was seen in both cell lines, and increased tumor necrosis factor- α was seen at 24 h for MB and 72 h for MCF-7 indicating increased inflammatory responses of the cancer cells. The two breast cancer celllines had different responses to MφCM, mainly involving inhibition rather than stimulation of growth of the cells, stimulation of senescence (MB cells) and increased inflammatory cytokine expression. The estrogen and progesterone receptor status of the cell lines may determine their response to MφCM. The function of the inflammatory cytokines in breast cancer growth remains to be identified.
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Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
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Conditioned medium from stimulated macrophages inhibits growth but induces an inflammatory phenotype in breast cancer cells
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- Simplified scoring system for prediction of mortal...
- Influence of tidal volume on ventilation distribut...
- Instructions to Authors
- Checklist
- Authorship and conflict statements
- Copyright transfer statement
- Permission request
- Ayurveda metallic-mineral 'Bhasma-associated sever...
- Phytobezoar and duodenal ulcer as complication of ...
- Just another case of bacterial meningitis... or......
- Ectopic insulinomas in the pelvis secondary to rec...
- Pineal gland apoplexy mimicking as migraine-like h...
- Unusual cause of elbow pain in a baseball pitcher
- Double tongue signs in a case of submandibular spa...
- Early-stage right temporal lobe variant of frontot...
- Pseudo-Wellens syndrome secondary to concurrent ca...
- Suction-induced retinopathy: optical coherence tom...
- Inflammatory myofibroblastic tumour: a rare cause ...
- Second look at Streptococcus sanguinis and the colon
- Atypical presentation of type B aortic dissection ...
- EBV colitis with ulcerative colitis: a double whammy
- Adult-onset epileptic aphasia
- Anomalous aortic origin of a coronary artery with ...
- Biots breathing associated with acute bacterial me...
- Subfoveal congenital hypertrophy of retinal pigmen...
- A case of massive haemoptysis
- Effectiveness of robo-assisted lower limb rehabili...
- A DNA nanostructured aptasensor for the sensitive ...
- An approach to the heating dynamics of residues fr...
- Impact of Regional Anesthesia on Gastroesophageal ...
- Metabolic Disorders and Critically Ill Patients
- Epidural Analgesia and Subcutaneous Heparin 3 Time...
- Proceedings From the Society for Advancement of Bl...
- Prospective Observational Investigation of Capnogr...
- Opioids for Acute Pain Management in Patients With...
- Practice of Ultrasound-Guided Palpation of Neck La...
- Dexmedetomidine Versus Remifentanil for Monitored ...
- Monitoring Depth of Hypnosis: Mid-Latency Auditory...
- Observation and Experiment: An Introduction to Cau...
- Remifentanil Stability
- Cervical Spine Motion During Tracheal Intubation U...
- Preoperative High-Dose Methylprednisolone and Glyc...
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- Protective Effect of Metformin Against Thyroid Can...
- American Thyroid Association Statement on Postoper...
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- Predicting early death in older adults with cancer
- Training for Medical Oncologists on Shared Decisio...
- Developing a Roadmap for Interventional Oncology
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- Occlusion, temporomandibular disorders, and orofac...
- Replacement of maxillary incisor crowns over disco...
- Comparison of marginal fit between CAD-CAM and hot...
- A digital technique for fabricating an interim imp...
- Retentive strength and marginal discrepancies of a...
- Use of finite element analysis for the assessment ...
- Adhesive evaluation of three types of resilient de...
- Osteogenesis ability of CAD-CAM biodegradable poly...
- Retention force and deformation of an innovative a...
- Accuracy of 3-dimensional computer-aided manufactu...
- Comparison of user satisfaction and image quality ...
- Load-bearing capacity of simulated Locator-retaine...
- In vitro retention of prefabricated and individual...
- Digital workflow to rehabilitate worn dentition on...
- Combination of digital photographs for the identif...
- Should the restoration of adjacent implants be spl...
- Fracture resistance and 2-body wear of 3-dimension...
- In vivo biocompatibility of an interim denture res...
- Digital workflow for the design and additively man...
- Comparison of fit accuracy of pressed lithium disi...
- Mast cells and basophils in allergic inflammation
- Seeing-and Nursing-Our Power.
- What RNs Can Do to Improve the Health of Their Com...
- HIV and Nurse Safety in China.
- Recognizing Lyme Disease.
- Simulation in Nursing Education.
- Joint Commission Issues Alert Addressing Violence ...
- Ireland Proposes Promising Model for Nurse Staffing.
- NewsCAP: The American Heart Association issues cor...
- The Impact of Legalization of Medical and Recreati...
- NewsCAP: Soldiers in the U.S. Army who deploy with...
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- State News Roundup.
- NewsCAP: Study finds that NPs, physicians, and phy...
- NewsCAP: The WHO releases Consolidated Guideline o...
- Can You Be Fired for Taking a Sick Day?.
- AJN On the Cover.
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- Reducing Fall Risk in Older Adults.
- Promoting Drug Safety in Older Adults.
- New HIV Drug Treats Patients Unresponsive to Other...
- Biomedical applications of functional peptides in ...
- Identification of loci where DNA methylation poten...
- Removal of bisphenol A by Fe-impregnated activated...
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