Publication date: Available online 28 June 2018
Source:Brain Research Bulletin
Author(s): Rui Lan, Ji-Tao Wu, Tao Wu, Yun-Zhi Ma, Bao-Qi Wang, Hai-Zhong Zheng, Ya-Na Li, Yan Wang, Chun-Qing Gu, Yong Zhang
This study examined the course of mitophagy following cerebral ischemia with reperfusion and the role of the PTEN-induced kinase 1 (PINK1)/Parkin/p62 signalling pathway. The middle cerebral artery of male Sprague-Dawley rats was occluded for 90 min and was followed by different time-points of reperfusion. Cerebral infarct areas were detected by 2,3,5-triphenyl tetrazolium chloride staining, while brain damage was observed by haematoxylin and eosin staining. Levels of LC3, Beclin1 and LAMP-1 were estimated by western blots. LC3B location was observed in various cells in the neurovascular unit. In addition, PINK1 accumulation in damaged mitochondria and Parkin/p62 mitochondrial translocation were investigated by double immunofluorescence staining. Finally, the levels of PINK1, Parkin and p62 expression in mitochondrial fractions were estimated by western blots. Cerebral ischemia with different time-points of reperfusion resulted in infarct in the territory of the middle cerebral artery accompanied by overall brain damage. In addition, we found up-regulation of LC3B, Beclin1, and LAMP-1, as well as mitophagy activation after reperfusion, with peak expression of these proteins at 24 h after reperfusion. Electron microscopy and immunofluorescence indicated that LC3B was primarily located in neurons, although lower levels of expression were found in astrocytes and even less in vascular endothelial cells. Moreover, significant increases in PINK1 accumulation in the outer membrane of mitochondria and increased Parkin/p62 mitochondrial translocation were shown at 24 h after reperfusion. These findings suggest that the PINK1/Parkin/p62 signalling pathway was involved in the pathophysiological processes following ischemia and reperfusion.
https://ift.tt/2yWp71y
Σφακιανάκης Αλέξανδρος
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5 Άγιος Νικόλαος
Κρήτη 72100
00302841026182
00306932607174
alsfakia@gmail.com
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
▼
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
-
▼
Ιουνίου
(5852)
-
▼
Ιουν 29
(200)
- Parvalbumin Interneurons Shape Neuronal Vulnerabil...
- Spreading of Tau Pathology in Sporadic Alzheimer’s...
- Effects of perineural invasion in prostate needle ...
- Simplified scoring system for prediction of mortal...
- Influence of tidal volume on ventilation distribut...
- Instructions to Authors
- Checklist
- Authorship and conflict statements
- Copyright transfer statement
- Permission request
- Ayurveda metallic-mineral 'Bhasma-associated sever...
- Phytobezoar and duodenal ulcer as complication of ...
- Just another case of bacterial meningitis... or......
- Ectopic insulinomas in the pelvis secondary to rec...
- Pineal gland apoplexy mimicking as migraine-like h...
- Unusual cause of elbow pain in a baseball pitcher
- Double tongue signs in a case of submandibular spa...
- Early-stage right temporal lobe variant of frontot...
- Pseudo-Wellens syndrome secondary to concurrent ca...
- Suction-induced retinopathy: optical coherence tom...
- Inflammatory myofibroblastic tumour: a rare cause ...
- Second look at Streptococcus sanguinis and the colon
- Atypical presentation of type B aortic dissection ...
- EBV colitis with ulcerative colitis: a double whammy
- Adult-onset epileptic aphasia
- Anomalous aortic origin of a coronary artery with ...
- Biots breathing associated with acute bacterial me...
- Subfoveal congenital hypertrophy of retinal pigmen...
- A case of massive haemoptysis
- Effectiveness of robo-assisted lower limb rehabili...
- A DNA nanostructured aptasensor for the sensitive ...
- An approach to the heating dynamics of residues fr...
- Impact of Regional Anesthesia on Gastroesophageal ...
- Metabolic Disorders and Critically Ill Patients
- Epidural Analgesia and Subcutaneous Heparin 3 Time...
- Proceedings From the Society for Advancement of Bl...
- Prospective Observational Investigation of Capnogr...
- Opioids for Acute Pain Management in Patients With...
- Practice of Ultrasound-Guided Palpation of Neck La...
- Dexmedetomidine Versus Remifentanil for Monitored ...
- Monitoring Depth of Hypnosis: Mid-Latency Auditory...
- Observation and Experiment: An Introduction to Cau...
- Remifentanil Stability
- Cervical Spine Motion During Tracheal Intubation U...
- Preoperative High-Dose Methylprednisolone and Glyc...
- Apparent Hyperthyroidism Caused by Biotin-Like Int...
- Sobetirome and its Amide Prodrug Sob-AM2 Exert Thy...
- Protective Effect of Metformin Against Thyroid Can...
- American Thyroid Association Statement on Postoper...
- Retraction of: Hair Iodine for Human Iodine Status...
- Structure of a Thyrotropin Receptor Monoclonal Ant...
- Predicting early death in older adults with cancer
- Training for Medical Oncologists on Shared Decisio...
- Developing a Roadmap for Interventional Oncology
- Fear of Mastectomy Associated with Delayed Breast ...
- Occlusion, temporomandibular disorders, and orofac...
- Replacement of maxillary incisor crowns over disco...
- Comparison of marginal fit between CAD-CAM and hot...
- A digital technique for fabricating an interim imp...
- Retentive strength and marginal discrepancies of a...
- Use of finite element analysis for the assessment ...
- Adhesive evaluation of three types of resilient de...
- Osteogenesis ability of CAD-CAM biodegradable poly...
- Retention force and deformation of an innovative a...
- Accuracy of 3-dimensional computer-aided manufactu...
- Comparison of user satisfaction and image quality ...
- Load-bearing capacity of simulated Locator-retaine...
- In vitro retention of prefabricated and individual...
- Digital workflow to rehabilitate worn dentition on...
- Combination of digital photographs for the identif...
- Should the restoration of adjacent implants be spl...
- Fracture resistance and 2-body wear of 3-dimension...
- In vivo biocompatibility of an interim denture res...
- Digital workflow for the design and additively man...
- Comparison of fit accuracy of pressed lithium disi...
- Mast cells and basophils in allergic inflammation
- Seeing-and Nursing-Our Power.
- What RNs Can Do to Improve the Health of Their Com...
- HIV and Nurse Safety in China.
- Recognizing Lyme Disease.
- Simulation in Nursing Education.
- Joint Commission Issues Alert Addressing Violence ...
- Ireland Proposes Promising Model for Nurse Staffing.
- NewsCAP: The American Heart Association issues cor...
- The Impact of Legalization of Medical and Recreati...
- NewsCAP: Soldiers in the U.S. Army who deploy with...
- NewsCAP: Each year an estimated 600 to 1,600 newbo...
- State News Roundup.
- NewsCAP: Study finds that NPs, physicians, and phy...
- NewsCAP: The WHO releases Consolidated Guideline o...
- Can You Be Fired for Taking a Sick Day?.
- AJN On the Cover.
- AJN On the Web.
- Reducing Fall Risk in Older Adults.
- Promoting Drug Safety in Older Adults.
- New HIV Drug Treats Patients Unresponsive to Other...
- Biomedical applications of functional peptides in ...
- Identification of loci where DNA methylation poten...
- Removal of bisphenol A by Fe-impregnated activated...
- S olubilization of insoluble zinc compounds by zin...
-
▼
Ιουν 29
(200)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
►
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
! # Ola via Alexandros G.Sfakianakis on Inoreader
Η λίστα ιστολογίων μου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(391)
- ► Φεβρουαρίου (200)
- ► Ιανουαρίου (191)
-
►
2022
(2843)
- ► Δεκεμβρίου (161)
- ► Σεπτεμβρίου (219)
- ► Φεβρουαρίου (264)
- ► Ιανουαρίου (280)
-
►
2021
(5625)
- ► Δεκεμβρίου (231)
- ► Σεπτεμβρίου (345)
- ► Φεβρουαρίου (620)
-
►
2020
(2065)
- ► Δεκεμβρίου (535)
- ► Σεπτεμβρίου (222)
- ► Φεβρουαρίου (28)
-
►
2019
(9608)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3791)
- ► Ιανουαρίου (3737)
-
▼
2018
(69720)
- ► Δεκεμβρίου (3507)
- ► Σεπτεμβρίου (3851)
-
▼
Ιουνίου
(5852)
-
▼
Ιουν 29
(200)
- Parvalbumin Interneurons Shape Neuronal Vulnerabil...
- Spreading of Tau Pathology in Sporadic Alzheimer’s...
- Effects of perineural invasion in prostate needle ...
- Simplified scoring system for prediction of mortal...
- Influence of tidal volume on ventilation distribut...
- Instructions to Authors
- Checklist
- Authorship and conflict statements
- Copyright transfer statement
- Permission request
- Ayurveda metallic-mineral 'Bhasma-associated sever...
- Phytobezoar and duodenal ulcer as complication of ...
- Just another case of bacterial meningitis... or......
- Ectopic insulinomas in the pelvis secondary to rec...
- Pineal gland apoplexy mimicking as migraine-like h...
- Unusual cause of elbow pain in a baseball pitcher
- Double tongue signs in a case of submandibular spa...
- Early-stage right temporal lobe variant of frontot...
- Pseudo-Wellens syndrome secondary to concurrent ca...
- Suction-induced retinopathy: optical coherence tom...
- Inflammatory myofibroblastic tumour: a rare cause ...
- Second look at Streptococcus sanguinis and the colon
- Atypical presentation of type B aortic dissection ...
- EBV colitis with ulcerative colitis: a double whammy
- Adult-onset epileptic aphasia
- Anomalous aortic origin of a coronary artery with ...
- Biots breathing associated with acute bacterial me...
- Subfoveal congenital hypertrophy of retinal pigmen...
- A case of massive haemoptysis
- Effectiveness of robo-assisted lower limb rehabili...
- A DNA nanostructured aptasensor for the sensitive ...
- An approach to the heating dynamics of residues fr...
- Impact of Regional Anesthesia on Gastroesophageal ...
- Metabolic Disorders and Critically Ill Patients
- Epidural Analgesia and Subcutaneous Heparin 3 Time...
- Proceedings From the Society for Advancement of Bl...
- Prospective Observational Investigation of Capnogr...
- Opioids for Acute Pain Management in Patients With...
- Practice of Ultrasound-Guided Palpation of Neck La...
- Dexmedetomidine Versus Remifentanil for Monitored ...
- Monitoring Depth of Hypnosis: Mid-Latency Auditory...
- Observation and Experiment: An Introduction to Cau...
- Remifentanil Stability
- Cervical Spine Motion During Tracheal Intubation U...
- Preoperative High-Dose Methylprednisolone and Glyc...
- Apparent Hyperthyroidism Caused by Biotin-Like Int...
- Sobetirome and its Amide Prodrug Sob-AM2 Exert Thy...
- Protective Effect of Metformin Against Thyroid Can...
- American Thyroid Association Statement on Postoper...
- Retraction of: Hair Iodine for Human Iodine Status...
- Structure of a Thyrotropin Receptor Monoclonal Ant...
- Predicting early death in older adults with cancer
- Training for Medical Oncologists on Shared Decisio...
- Developing a Roadmap for Interventional Oncology
- Fear of Mastectomy Associated with Delayed Breast ...
- Occlusion, temporomandibular disorders, and orofac...
- Replacement of maxillary incisor crowns over disco...
- Comparison of marginal fit between CAD-CAM and hot...
- A digital technique for fabricating an interim imp...
- Retentive strength and marginal discrepancies of a...
- Use of finite element analysis for the assessment ...
- Adhesive evaluation of three types of resilient de...
- Osteogenesis ability of CAD-CAM biodegradable poly...
- Retention force and deformation of an innovative a...
- Accuracy of 3-dimensional computer-aided manufactu...
- Comparison of user satisfaction and image quality ...
- Load-bearing capacity of simulated Locator-retaine...
- In vitro retention of prefabricated and individual...
- Digital workflow to rehabilitate worn dentition on...
- Combination of digital photographs for the identif...
- Should the restoration of adjacent implants be spl...
- Fracture resistance and 2-body wear of 3-dimension...
- In vivo biocompatibility of an interim denture res...
- Digital workflow for the design and additively man...
- Comparison of fit accuracy of pressed lithium disi...
- Mast cells and basophils in allergic inflammation
- Seeing-and Nursing-Our Power.
- What RNs Can Do to Improve the Health of Their Com...
- HIV and Nurse Safety in China.
- Recognizing Lyme Disease.
- Simulation in Nursing Education.
- Joint Commission Issues Alert Addressing Violence ...
- Ireland Proposes Promising Model for Nurse Staffing.
- NewsCAP: The American Heart Association issues cor...
- The Impact of Legalization of Medical and Recreati...
- NewsCAP: Soldiers in the U.S. Army who deploy with...
- NewsCAP: Each year an estimated 600 to 1,600 newbo...
- State News Roundup.
- NewsCAP: Study finds that NPs, physicians, and phy...
- NewsCAP: The WHO releases Consolidated Guideline o...
- Can You Be Fired for Taking a Sick Day?.
- AJN On the Cover.
- AJN On the Web.
- Reducing Fall Risk in Older Adults.
- Promoting Drug Safety in Older Adults.
- New HIV Drug Treats Patients Unresponsive to Other...
- Biomedical applications of functional peptides in ...
- Identification of loci where DNA methylation poten...
- Removal of bisphenol A by Fe-impregnated activated...
- S olubilization of insoluble zinc compounds by zin...
-
▼
Ιουν 29
(200)
- ► Φεβρουαρίου (8116)
- ► Ιανουαρίου (7758)
-
►
2017
(111579)
- ► Δεκεμβρίου (7718)
- ► Σεπτεμβρίου (7549)
- ► Φεβρουαρίου (10753)
- ► Ιανουαρίου (10529)
-
►
2016
(16402)
- ► Δεκεμβρίου (7478)
- ► Φεβρουαρίου (900)
- ► Ιανουαρίου (1250)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου