Single-cell epigenetics – Chromatin modification atlas unveiled by mass cytometry

Publication date: Available online 28 June 2018
Source:Clinical Immunology
Author(s): Peggie Cheung, Francesco Vallania, Mai Dvorak, Sarah E. Chang, Steven Schaffert, Michele Donato, Aditya Rao, Rong Mao, Paul J. Utz, Purvesh Khatri, Alex J. Kuo
Modifications of histone proteins are fundamental to the regulation of epigenetic phenotypes. Dysregulations of histone modifications have been linked to the pathogenesis of diverse human diseases. However, identifying differential histone modifications in patients with immune-mediated diseases has been challenging, in part due to the lack of a powerful analytic platform to study histone modifications in the complex human immune system. We recently developed a highly multiplexed platform, Epigenetic landscape profiling using cytometry by Time-Of-Flight (EpiTOF), to analyze the global levels of a broad array of histone modifications in single cells using mass cytometry. In this review, we summarize the development of EpiTOF and discuss its potential applications in biomedical research. We anticipate that this platform will provide new insights into the roles of epigenetic regulation in hematopoiesis, immune cell functions and immune system aging, and reveal aberrant epigenetic patterns associated with immune-mediated diseases.



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