TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: association with apical periodontitis

alexandrossfakianakis shared this article with you from Inoreader TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: association with apical periodontitis via International Endodontic Journal Abstract Aim The aim of this case-control study was to evaluate the association between the TNFSF13B rs9514828 (-871 C>T) polymorphism and soluble BAFF (sBAFF) in apical periodontitis (AP) patients. Methodology 261 healthy subjects (HS) and 158 patients with AP classified as: 46 acute apical abscess (AAA), 81 primary AP (pAP) and 31 secondary AP (sAP) patients were included. Genomic DNA (gDNA) was extracted from peripheral blood cells according to the salting-out method. The TNFSF13B rs9514828 (NC_000013.11:g.108269025C>T) were identified using polymerase chain reaction (PCR) followed by restriction fragment length polymorphisms (RFLP). Serum sBAFF levels were measured by ELISA test. The chi-square or Fisher's exact test was performed. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated to evaluate the risk of AP associated with the rs9514828. The Mann–Whitney U test and Kruskal–Wallis analysis were used for non- normally distributed data. Differences were considered significant with a P-value < 0.05. Results No differences in the genotype/ allele frequencies were shown between HS and patients with AAA. However, the TT genotype (OR= 2.68, 95%CI: 1.10-6.53; P = 0.025) and T allele (OR= 1.46, 95%CI: 1.00-2.12; P = 0.045) were associated with increased risk of pAP. In contrast, the minor allele T significantly decreased the risk of sAP (OR = 0.49, 95%CI: 0.024-0.99; P = 0.043). sBAFF serum levels were increased in AAA and pAP compared with HS (P < 0.01 and P = 0.021, respectively). The AAA patients had higher sBAFF serum levels than pAP (P = 0.034) and sAP (P < 0.01). Conclusions These results suggest that the TNFSF13B rs9514828 (-871 C>T) polymorphism is associated with pAP susceptibility and that BAFF is a cytokine that might be involved in acute and chronic AP. The future exploration of the rs9514828 polymorphism in other AP cohorts is recommended. View on Web

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