Cu(2+)-attached pumice particles embedded composite cryogels for protein purification.

Cu(2+)-attached pumice particles embedded composite cryogels for protein purification.

Artif Cells Nanomed Biotechnol. 2016 Jan 11;:1-8

Authors: Alkan H, Cömert ŞC, Gürbüz F, Doğru M, Odabaşı M

Abstract
In this study, chromatographic performance of Cu(2+)-attached pumice particles embedded to monolithic cryogels (Cu(2+)-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.

PMID: 26755205 [PubMed - as supplied by publisher]

from #PubMed 1 via ola Kala on Inoreader http://ift.tt/1PbbW8f
via IFTTT

from #Med Blogs by Alexandros G.Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1mV7Jil
via IFTTT