Flow cytometric data analysis of circulating progenitor cell stability

Publication date: Available online 7 December 2016
Source:Data in Brief
Author(s): Ernestine A. Mahar, Liping Mou, Salim S. Hayek, Arshed A. Quyyumi, Edmund K. Waller
We performed a quality control assessment of the stability of circulating blood progenitor cells in blood samples stored at 4°C, to determine the time period during which blood samples can be analyzed and yield consistent data for progenitor cell content. Healthy volunteers (n=6) were recruited and underwent phlebotomy, and blood was stored in EDTA tubes at 4°C. Flow cytometry was performed to quantitate progenitor cell subsets at 0–4h, 24h, and 48h post phlebotomy. All processed samples were fixed with 1% Paraformaldehyde and 1,000,000 total data events were collected. We found no significant differences in PC data for both CD34+ (P=0.68 for one-way ANOVA) and CD34+/CD133+ (P=0.74 for one-way ANOVA).



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