Preliminary research on DNA methylation changes during murine palatogenesis induced by TCDD

2,3,7,8-tetrachlrodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate through growth factor and receptor expression changes during palatogenesis. DNA methylation is an important epigenetic modification that can regulate gene expressions and may be involved in TCDD-induced cleft palate. In this study, we investigated the effects of TCDD on the global and CpG DNA methylation status and the expression levels of DNA methyltransferases(Dnmts) in palate tissue of fetal mice. Pregnant C57BL/6J mice were administered with corn oil or TCDD 28μg/kg at gestation day 10.5(GD10.5), and sacrificed at GD13.5,14.5,15.5.Fetal palates were collected for molecular analysis.

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